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Consistently, our T-maze test also exhibited significant improvement in the performance of 5XFAD, as shown by increase in the number of positive turns (Figure 6F) and reduction of errors (Figure 6G), whereas 5XFAD Ppar-null mice and 5XFAD Ppar-Hippo mice did not display any improvement in T maze analyses (Figure 6, F and G), following Hex treatment, indicating a pivotal role of hippocampal PPAR in Hex-mediated improvement in spatial and contextual in 5XFAD mice

Demand outstripped supply

Samples were reduced with DTT (5 L of 200 mM stock in H 2 O, 10 mM final concentration) and incubated at 65 C for 15 min, then alkylated with iodoacetamide (5 L of 400 mM stock in H 2 O, 20 mM final concentration) and shaken at 37 C for 30 min in the dark

Br J Pharmacol 1996;118:14611468.ArticlePubMedPMC 34

If a step is rough, the trial protocols themselves allowed holding it for an extra 4 weeks or dropping back one level